multiplex pcr assay kit v 2 Search Results


nebnext rrna depletion kit  (New England Biolabs)
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New England Biolabs nebnext rrna depletion kit
Nebnext Rrna Depletion Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e7335 trueprep dna library prep kit v2 for illumina vazyme biotech  (Vazyme Biotech Co)
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Vazyme Biotech Co e7335 trueprep dna library prep kit v2 for illumina vazyme biotech
E7335 Trueprep Dna Library Prep Kit V2 For Illumina Vazyme Biotech, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nebnext rrna depletion kit v2  (New England Biolabs)
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New England Biolabs nebnext rrna depletion kit v2
A. gRNAs designed for METTL5 knockout targeting the first exon with a 110 bp deletion that causes a frameshift and disrupts the ORF. B. qPCR validation of METTL5 KO in two distinct KO clones for each KOLF2.1J, H9, and AICS90 pluripotent stem cell lines shows loss of METTL5 transcript in KOs; **** p<0.0001. C. Western blot confirmation of METTL5 protein loss in all KO cell lines. D. m 6 A ELISA shows significant reduction in m 6 A from twice purified 40-nt fragments of 18S <t>rRNA</t> containing A 1832 . n=3 replicates; ***p<0.001. E. Cortical forebrain organoid differentiation protocol starting from hiPSCs and growing for 75 days. F-H. DAPI-stained KOLF2.1J Control and METTL5- KO organoids at Days 40, 55, and 75 show significant differences in organoid diameter and the number of ventricle-like structures per organoid. n=5-8 organoids/timepoint for control and KO; ns= no significance, *p<0.05, ** p<0.01, ***p<0.001, ****p<0.0001.
Nebnext Rrna Depletion Kit V2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiplex+pcr+assay+kit+v+2/NEBNext+rRNA+Depletion+Kit+v2/bio_rxiv__2025__07__13__664555-308-21-27
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nebnext rrna depletion kit v2 - by Bioz Stars, 2026-09
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10x genomics chromium  (10X Genomics)
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10X Genomics 10x genomics chromium
A. gRNAs designed for METTL5 knockout targeting the first exon with a 110 bp deletion that causes a frameshift and disrupts the ORF. B. qPCR validation of METTL5 KO in two distinct KO clones for each KOLF2.1J, H9, and AICS90 pluripotent stem cell lines shows loss of METTL5 transcript in KOs; **** p<0.0001. C. Western blot confirmation of METTL5 protein loss in all KO cell lines. D. m 6 A ELISA shows significant reduction in m 6 A from twice purified 40-nt fragments of 18S <t>rRNA</t> containing A 1832 . n=3 replicates; ***p<0.001. E. Cortical forebrain organoid differentiation protocol starting from hiPSCs and growing for 75 days. F-H. DAPI-stained KOLF2.1J Control and METTL5- KO organoids at Days 40, 55, and 75 show significant differences in organoid diameter and the number of ventricle-like structures per organoid. n=5-8 organoids/timepoint for control and KO; ns= no significance, *p<0.05, ** p<0.01, ***p<0.001, ****p<0.0001.
10x Genomics Chromium, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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10x genomics chromium - by Bioz Stars, 2026-09
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v2 300 cycle flow cell  (Illumina Inc)
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Illumina Inc v2 300 cycle flow cell
A. gRNAs designed for METTL5 knockout targeting the first exon with a 110 bp deletion that causes a frameshift and disrupts the ORF. B. qPCR validation of METTL5 KO in two distinct KO clones for each KOLF2.1J, H9, and AICS90 pluripotent stem cell lines shows loss of METTL5 transcript in KOs; **** p<0.0001. C. Western blot confirmation of METTL5 protein loss in all KO cell lines. D. m 6 A ELISA shows significant reduction in m 6 A from twice purified 40-nt fragments of 18S <t>rRNA</t> containing A 1832 . n=3 replicates; ***p<0.001. E. Cortical forebrain organoid differentiation protocol starting from hiPSCs and growing for 75 days. F-H. DAPI-stained KOLF2.1J Control and METTL5- KO organoids at Days 40, 55, and 75 show significant differences in organoid diameter and the number of ventricle-like structures per organoid. n=5-8 organoids/timepoint for control and KO; ns= no significance, *p<0.05, ** p<0.01, ***p<0.001, ****p<0.0001.
V2 300 Cycle Flow Cell, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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v2 300 cycle flow cell - by Bioz Stars, 2026-09
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illumina 20024907  (Illumina Inc)
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Illumina Inc illumina 20024907
A. gRNAs designed for METTL5 knockout targeting the first exon with a 110 bp deletion that causes a frameshift and disrupts the ORF. B. qPCR validation of METTL5 KO in two distinct KO clones for each KOLF2.1J, H9, and AICS90 pluripotent stem cell lines shows loss of METTL5 transcript in KOs; **** p<0.0001. C. Western blot confirmation of METTL5 protein loss in all KO cell lines. D. m 6 A ELISA shows significant reduction in m 6 A from twice purified 40-nt fragments of 18S <t>rRNA</t> containing A 1832 . n=3 replicates; ***p<0.001. E. Cortical forebrain organoid differentiation protocol starting from hiPSCs and growing for 75 days. F-H. DAPI-stained KOLF2.1J Control and METTL5- KO organoids at Days 40, 55, and 75 show significant differences in organoid diameter and the number of ventricle-like structures per organoid. n=5-8 organoids/timepoint for control and KO; ns= no significance, *p<0.05, ** p<0.01, ***p<0.001, ****p<0.0001.
Illumina 20024907, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiplex+pcr+assay+kit+v+2/NextSeq+500%2F550+High+Output+Kit+v2%2E5/pm39127040-236-180-180
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truseq rna library preparation kit v2  (Illumina Inc)
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Illumina Inc truseq rna library preparation kit v2
A. gRNAs designed for METTL5 knockout targeting the first exon with a 110 bp deletion that causes a frameshift and disrupts the ORF. B. qPCR validation of METTL5 KO in two distinct KO clones for each KOLF2.1J, H9, and AICS90 pluripotent stem cell lines shows loss of METTL5 transcript in KOs; **** p<0.0001. C. Western blot confirmation of METTL5 protein loss in all KO cell lines. D. m 6 A ELISA shows significant reduction in m 6 A from twice purified 40-nt fragments of 18S <t>rRNA</t> containing A 1832 . n=3 replicates; ***p<0.001. E. Cortical forebrain organoid differentiation protocol starting from hiPSCs and growing for 75 days. F-H. DAPI-stained KOLF2.1J Control and METTL5- KO organoids at Days 40, 55, and 75 show significant differences in organoid diameter and the number of ventricle-like structures per organoid. n=5-8 organoids/timepoint for control and KO; ns= no significance, *p<0.05, ** p<0.01, ***p<0.001, ****p<0.0001.
Truseq Rna Library Preparation Kit V2, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiplex+pcr+assay+kit+v+2/TruSeq+RNA+Library+Preparation+Kit+v2/pm34287647-332-9-8
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xt index kit v2  (Illumina Inc)
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Illumina Inc xt index kit v2
A. gRNAs designed for METTL5 knockout targeting the first exon with a 110 bp deletion that causes a frameshift and disrupts the ORF. B. qPCR validation of METTL5 KO in two distinct KO clones for each KOLF2.1J, H9, and AICS90 pluripotent stem cell lines shows loss of METTL5 transcript in KOs; **** p<0.0001. C. Western blot confirmation of METTL5 protein loss in all KO cell lines. D. m 6 A ELISA shows significant reduction in m 6 A from twice purified 40-nt fragments of 18S <t>rRNA</t> containing A 1832 . n=3 replicates; ***p<0.001. E. Cortical forebrain organoid differentiation protocol starting from hiPSCs and growing for 75 days. F-H. DAPI-stained KOLF2.1J Control and METTL5- KO organoids at Days 40, 55, and 75 show significant differences in organoid diameter and the number of ventricle-like structures per organoid. n=5-8 organoids/timepoint for control and KO; ns= no significance, *p<0.05, ** p<0.01, ***p<0.001, ****p<0.0001.
Xt Index Kit V2, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
xt index kit v2 - by Bioz Stars, 2026-09
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rnascope intro pack for multiplex fluorescent reagent kit v2- mm  (Advanced Cell Diagnostics Inc)
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Advanced Cell Diagnostics Inc rnascope intro pack for multiplex fluorescent reagent kit v2- mm
A. gRNAs designed for METTL5 knockout targeting the first exon with a 110 bp deletion that causes a frameshift and disrupts the ORF. B. qPCR validation of METTL5 KO in two distinct KO clones for each KOLF2.1J, H9, and AICS90 pluripotent stem cell lines shows loss of METTL5 transcript in KOs; **** p<0.0001. C. Western blot confirmation of METTL5 protein loss in all KO cell lines. D. m 6 A ELISA shows significant reduction in m 6 A from twice purified 40-nt fragments of 18S <t>rRNA</t> containing A 1832 . n=3 replicates; ***p<0.001. E. Cortical forebrain organoid differentiation protocol starting from hiPSCs and growing for 75 days. F-H. DAPI-stained KOLF2.1J Control and METTL5- KO organoids at Days 40, 55, and 75 show significant differences in organoid diameter and the number of ventricle-like structures per organoid. n=5-8 organoids/timepoint for control and KO; ns= no significance, *p<0.05, ** p<0.01, ***p<0.001, ****p<0.0001.
Rnascope Intro Pack For Multiplex Fluorescent Reagent Kit V2 Mm, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rnascope intro pack for multiplex fluorescent reagent kit v2- mm - by Bioz Stars, 2026-09
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rnascope multiplex fluorescent detection kit v2  (Advanced Cell Diagnostics Inc)
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Advanced Cell Diagnostics Inc rnascope multiplex fluorescent detection kit v2
A. gRNAs designed for METTL5 knockout targeting the first exon with a 110 bp deletion that causes a frameshift and disrupts the ORF. B. qPCR validation of METTL5 KO in two distinct KO clones for each KOLF2.1J, H9, and AICS90 pluripotent stem cell lines shows loss of METTL5 transcript in KOs; **** p<0.0001. C. Western blot confirmation of METTL5 protein loss in all KO cell lines. D. m 6 A ELISA shows significant reduction in m 6 A from twice purified 40-nt fragments of 18S <t>rRNA</t> containing A 1832 . n=3 replicates; ***p<0.001. E. Cortical forebrain organoid differentiation protocol starting from hiPSCs and growing for 75 days. F-H. DAPI-stained KOLF2.1J Control and METTL5- KO organoids at Days 40, 55, and 75 show significant differences in organoid diameter and the number of ventricle-like structures per organoid. n=5-8 organoids/timepoint for control and KO; ns= no significance, *p<0.05, ** p<0.01, ***p<0.001, ****p<0.0001.
Rnascope Multiplex Fluorescent Detection Kit V2, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiplex+pcr+assay+kit+v+2/RNAscope+Multiplex+Fluorescent+Detection+Kit+v2/custom%40323110%4010%2E1158%2F2159-8290%2Ecd-24-1853
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rnascope multiplex fluorescent reagent kit v2  (Advanced Cell Diagnostics Inc)
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Advanced Cell Diagnostics Inc rnascope multiplex fluorescent reagent kit v2
A. gRNAs designed for METTL5 knockout targeting the first exon with a 110 bp deletion that causes a frameshift and disrupts the ORF. B. qPCR validation of METTL5 KO in two distinct KO clones for each KOLF2.1J, H9, and AICS90 pluripotent stem cell lines shows loss of METTL5 transcript in KOs; **** p<0.0001. C. Western blot confirmation of METTL5 protein loss in all KO cell lines. D. m 6 A ELISA shows significant reduction in m 6 A from twice purified 40-nt fragments of 18S <t>rRNA</t> containing A 1832 . n=3 replicates; ***p<0.001. E. Cortical forebrain organoid differentiation protocol starting from hiPSCs and growing for 75 days. F-H. DAPI-stained KOLF2.1J Control and METTL5- KO organoids at Days 40, 55, and 75 show significant differences in organoid diameter and the number of ventricle-like structures per organoid. n=5-8 organoids/timepoint for control and KO; ns= no significance, *p<0.05, ** p<0.01, ***p<0.001, ****p<0.0001.
Rnascope Multiplex Fluorescent Reagent Kit V2, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiplex+pcr+assay+kit+v+2/RNAscope+Multiplex+Fluorescent+Reagent+Kit+v2/custom%40323100%4010%2E64898%2F2026%2E06%2E15%2E732400
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e7765 nebnext rrna depletion kit v2 new england biolabs  (New England Biolabs)
98
New England Biolabs e7765 nebnext rrna depletion kit v2 new england biolabs
A. gRNAs designed for METTL5 knockout targeting the first exon with a 110 bp deletion that causes a frameshift and disrupts the ORF. B. qPCR validation of METTL5 KO in two distinct KO clones for each KOLF2.1J, H9, and AICS90 pluripotent stem cell lines shows loss of METTL5 transcript in KOs; **** p<0.0001. C. Western blot confirmation of METTL5 protein loss in all KO cell lines. D. m 6 A ELISA shows significant reduction in m 6 A from twice purified 40-nt fragments of 18S <t>rRNA</t> containing A 1832 . n=3 replicates; ***p<0.001. E. Cortical forebrain organoid differentiation protocol starting from hiPSCs and growing for 75 days. F-H. DAPI-stained KOLF2.1J Control and METTL5- KO organoids at Days 40, 55, and 75 show significant differences in organoid diameter and the number of ventricle-like structures per organoid. n=5-8 organoids/timepoint for control and KO; ns= no significance, *p<0.05, ** p<0.01, ***p<0.001, ****p<0.0001.
E7765 Nebnext Rrna Depletion Kit V2 New England Biolabs, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A. gRNAs designed for METTL5 knockout targeting the first exon with a 110 bp deletion that causes a frameshift and disrupts the ORF. B. qPCR validation of METTL5 KO in two distinct KO clones for each KOLF2.1J, H9, and AICS90 pluripotent stem cell lines shows loss of METTL5 transcript in KOs; **** p<0.0001. C. Western blot confirmation of METTL5 protein loss in all KO cell lines. D. m 6 A ELISA shows significant reduction in m 6 A from twice purified 40-nt fragments of 18S rRNA containing A 1832 . n=3 replicates; ***p<0.001. E. Cortical forebrain organoid differentiation protocol starting from hiPSCs and growing for 75 days. F-H. DAPI-stained KOLF2.1J Control and METTL5- KO organoids at Days 40, 55, and 75 show significant differences in organoid diameter and the number of ventricle-like structures per organoid. n=5-8 organoids/timepoint for control and KO; ns= no significance, *p<0.05, ** p<0.01, ***p<0.001, ****p<0.0001.

Journal: bioRxiv

Article Title: Cortical organoids reveal human-specific roles of METTL5 in neurodevelopment via regulation of CHCHD2

doi: 10.1101/2025.07.13.664555

Figure Lengend Snippet: A. gRNAs designed for METTL5 knockout targeting the first exon with a 110 bp deletion that causes a frameshift and disrupts the ORF. B. qPCR validation of METTL5 KO in two distinct KO clones for each KOLF2.1J, H9, and AICS90 pluripotent stem cell lines shows loss of METTL5 transcript in KOs; **** p<0.0001. C. Western blot confirmation of METTL5 protein loss in all KO cell lines. D. m 6 A ELISA shows significant reduction in m 6 A from twice purified 40-nt fragments of 18S rRNA containing A 1832 . n=3 replicates; ***p<0.001. E. Cortical forebrain organoid differentiation protocol starting from hiPSCs and growing for 75 days. F-H. DAPI-stained KOLF2.1J Control and METTL5- KO organoids at Days 40, 55, and 75 show significant differences in organoid diameter and the number of ventricle-like structures per organoid. n=5-8 organoids/timepoint for control and KO; ns= no significance, *p<0.05, ** p<0.01, ***p<0.001, ****p<0.0001.

Article Snippet: 1-2 ug of RNA was purified using the Direct-zol RNA miniprep kit (Zymo) then used in RNA seq library prep kits: NEBNext rRNA Depletion Kit V2 (human/mouse/rat) (NEB), NEBNext Ultra II Directional RNA Library Prep Kit of Illumina (E7760), with NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (E6440S)

Techniques: Knock-Out, Biomarker Discovery, Clone Assay, Western Blot, Enzyme-linked Immunosorbent Assay, Purification, Staining, Control